The main content of biochemistry contains the basic chemical reactions related to biology, structures of bio-macromolecules, enzyme catalytic reactions, energy metabolism, processes, and mechanisms of signal transduction. The course also focuses on the catabolism and anabolism pathways of sugars, lipids, proteins, and nucleic acids, which are essential for the maintenance of physiological functions. Understanding these metabolic pathways is not only for revealing the causes of metabolic diseases but also helpful for the development of healthy food and medicine.
結構生物學課程之目的為探討蛋白質的結構與功能的相關性，並介紹各種結構測定方法，如X-ray crystallography、NMR、SAXS與Cryo-EM。課程的最終目標是想讓學生培養出分析結構的能力，能夠根據蛋白質結構所提供資訊息，推測出蛋白質的功能，並設計實驗去印證自己的假設。此外，此課程也會介紹結構生物學的應用並教授結構分析軟體 (Pymol、spdbv與Chimera) 的使用。
The purpose of structural biology is to understand the relevance between protein structure and function. In this course, we introduce the methods for structure determination, such as X-ray crystallography, NMR, SAXS, and Cryo-EM. The final destination of this course is training students’ ability to analyzing protein structure, figuring out protein function according to information from protein structure, and designing an experiment to prove their hypothesis. Besides, this course will also introduce the applications of structural biology and how to use structure analyzing software (ex. Pymol, Spdbv, and Chimera).
分子生物學為現代生物學重要的基石，其所學圍繞著生物體中基因與遺傳訊息表現的中心法則(central dogma)，探討著體內的基因與遺傳訊息是以何種方式去調控並表達。換句話說，分子生物學是涵蓋了由DNA轉錄成RNA再從RNA轉譯成蛋白質的整體過程，以及在此過程中受到何種生物巨分子的正向與負向調控，看似簡單的法則其中卻也蘊含許多博大精深且複雜的知識。除此之外，課堂中也會介紹分子生物學中的各種基礎與進階實驗的原理以及步驟。在這堂課中，蕭育源老師負責上學期的分子生物學的整體概述、基因組的特性以及各種分子生物學實驗方法的介紹，下學期則是負責RNA剪接(RNA splicing)、RNA生成、降解與相關的調控。教學評鑑的學生反應問卷請點下方的學生反饋。
Molecular biology is an essential cornerstone of modern biology, which surrounds central dogma and is used to understand how genes and genetic messages in the body are regulated and expressed. In other words, it refers to the processes of transcription from DNA into RNA and then translation from RNA to protein, and the positive and negative regulation of biological macromolecules. The seemingly simple rules also contain much profound and convoluted knowledge. Besides, the principles and steps of various primary and advanced experiments in molecular biology will be introduced in the class.
Proteins and nucleic acids are essential bio-macromolecules in cells. How they interact together and catalysis are hot issues for biologists. This course provides techniques about studying nucleic acid-binding proteins, including in vivo, in vitro experiments, and structural approaches. Besides, we will discuss the structures and functions of distinct nucleic acid-binding proteins.
The course of “protein expression, purification, crystallization, and structure analysis” is equal to bio-macromolecular crystallography, which is a significant subject in structural biology. The main content of the course is to introduce how to resolve three-dimensional structures of bio-macromolecules by crystallography and X-ray diffraction techniques. This course will thoroughly introduce the concepts and experimental processes from cloning, protein expression to structure determination, giving students an insight into the procedures and principles of three-dimensional structure determination.
Biotech-Art is a course that uses biotechnology-related materials for artistic creation, including images, readily available animal and plant materials, biotechnology experimental consumables, 3D printing models, and dance, etc. Our artworks will be used to discuss issues arising from the development of biotechnology, such as the welfare of experimental animals, genetically modified organisms, the regulation of artificial organs and food, or the moral conflicts of using human embryos as experimental materials. Let people understand the good and evil, cruelty and convenience, beauty and sadness of biotechnology. The class's content includes three parts: the teacher's lecture, group discussion, and collective creation. We hope this class can awaken the love of biology and technology in the hearts of students.
The course is to bring information on how to give an interesting presentation in English with slides in 30min. Group discussion and questions raised are required. Prof. Hsiao will also help students understand the types of research articles and their submission process, journal reading skills, presentation skills, and reference management software-related knowledge. If there is extra time, how to write abstracts accurately will also be involved.